Fig. 3
From: Olfactory bulb anomalies in KBG syndrome mouse model and patients
Loss of Ankrd11 in E14.5 NPCs leads to abrogated P15 RMS migration. Also see Supplemental Fig. 2. A Ankrd11fl/fl;RosaYFPSTOP/STOP dams mated with Ankrd11fl/fl;RosaYFPSTOP/STOP;NestinCreERT2 males were injected with TMX at E14.5. Resulting mice (Ankrd11control and Ankrd11nscKO) were collected at P15. Images were collected from anatomically matched medial (M) and lateral (L) sagittal sections encompassing OB. RMS indicated in green. B Schematic of areas measured in P15 RMS analysis. Region 1 encompassed the caudodorsal (CD) zone in the RMS (indicated by a blue arrowhead), region 2 the ventral intermediate zone in the RMS (indicated by a red arrow), and region 3 where the RMS meets the OB (indicated by a yellow star). C, D Representative images from Ankrd11control and Ankrd11nscKO P15 medial (C) and lateral (D) OB encompassing RMS immunostained for DCX (green) and Hoechst (white). Blue arrowhead indicates CD RMS (1), red arrow indicates VI RMS (2), yellow star indicates OB (3). White dashed boxes in lateral Hoechst panel in D are shown in the right column at higher magnification and labeled with regions #1–3 corresponding the regions identified in B. E Quantification of integrated density of Hoechst signal in CD RMS (1), VI RMS (2) and OB (3) regions outlined in D. F Quantification of region #1 (CD RMS) area. Error bars represent SEM. Data was analyzed using unpaired t-test. ns not significant, *p < 0.05, **p < 0.01, ***p < 0.001. n = 4–5 mice per genotype from at least two independent litters. Scale bars represent 1000 μm (C, D), 200 μm (insets of D). E embryonic day, L lateral, LV lateral ventricle, M medial, OB olfactory bulb, RMS rostral migratory stream