Fig. 2
From: Olfactory bulb anomalies in KBG syndrome mouse model and patients
Loss of Ankrd11 in E14.5 NPCs results in reduced P15 OB with aberrant cellular composition. Also see Supplemental Fig. 1. A Ankrd11fl/fl;RosaYFPSTOP/STOP dams mated with Ankrd11fl/fl;RosaYFPSTOP/STOP;NestinCreERT2 males were injected with TMX at E14.5. Resulting pups (Ankrd11control and Ankrd11nscKO) were collected at P15. Images were collected from anatomically matched medial (M) and lateral (L) sagittal sections encompassing OB. B Representative images of Ankrd11control and Ankrd11nscKO sagittal sections stained with Hoechst (white). Solid outline shows OB area (excluding ONL) of Ankrd11control (yellow) and Ankrd11nscKO (red). C Representative overlap of Ankrd11control and Ankrd11nscKO OB area from (B). D Quantification of B. E Representative P15 OB schematic that displays identification of OB center by circle and crosshairs method followed by demarcation of the OB column and layers. Please see “Materials and methods” for more details. RMS rostral migratory stream, GL glomerular layer, MCL mitral cell layer, EPL external plexiform layer, GCL granule cell layer. F, G Representative images of Ankrd11control (F) and Ankrd11nscKO (G) OB stained with Hoechst (white), blue crosshair shows center of OB used to demarcate the OB column (green rectangle). OB column crop is on the right side with demarcated layers. H Quantification of OB layer depth shown in F and G. I–T Representative images and quantification of marker + cells in Ankrd11control and Ankrd11nscKO OB column layers immunostained for DCX (green) (I, J), NEUN (red) (K, L), CALB (green) (M, N), NG (pink) (O, P), GFAP (red) (Q, R), and PDGFRα (yellow) (S, T). Hoechst is counterstained in blue. The Hoechst panels in I, Q, and S were obtained from the same image immunostained for DCX, GFAP, and PDGFRα; and in K and M from the same image immunostained for NEUN and CALB. Error bars represent SEM. Data was analyzed using two-way ANOVA followed by Tukey’s multiple comparisons post hoc test. n = 5 mice per genotype from at least two independent litters. Scale bars represent 1000 μm (B), 500 μm (F and G), 100 μm (I, K, M, O, Q and S). CB cerebellum, Ctx cortex, E embryonic day, L lateral, M medial, OB olfactory bulb, P postnatal day